Fluorescent In Situ Hybridization Probe Design

Fluorescence in situ hybridization fish is a technique that uses fluorescent probes which bind to special sites of the chromosome with a high degree of sequence complementarity to the probes.

Fluorescent in situ hybridization probe design.

Curr opin biotechnol. Where the insert may contain a specific gene or originate from a specific chromosomal. Multiplex fluorescence in situ hybridization fish enables you to assay multiple targets and visualize colocalized signals in a single specimen. Fluorescence in situ hybridization fish of genes and mrna is most often based on polynucleotide probes.

Fluorescence in situ hybridization fish is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of a nucleic acid sequence with a high degree of sequence complementarity it was developed by biomedical researchers in the early 1980s to detect and localize the presence or absence of specific dna sequences on chromosomes. However the well established concepts for oligonucleotide probe design cannot be transferred to polynucleotides. Wagner m haider s. The dna probe typically comes from cloned sources such as plasmids cosmids pacs yacs or bacs.

Fluorescence in situ hybridization fish involves the preparation of two main components. Using spectrally distinct fluorophore labels for each hybridization probe this approach gives you the power to resolve several genetic elements or multiple gene expression patterns through multicolor visual display. The dna probe and the target dna to which the probe will be hybridized.